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Fig. 4

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ZDB-FIG-100622-9

Publication

Devireddy et al., 2010 - A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production

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Fig. 4

BDH2 Is Required to Maintain Normal Cytoplasmic Iron Levels

FL5.12 cells expressing a Bdh2 or control shRNA were analyzed for total cytoplasmic iron levels by colorimetric analysis (A), for free cytoplasmic iron concentration by fluorescence calcein assay (B), for ferritin-L (C) and TFR1 and IRP2 (D) levels by immunoblot analysis, and for cytosolic aconitase activity (E). Error bars indicate the SD. See also Figures S3A–S3D and S4.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell