ZFIN Image: Devireddy et al., 2010, Fig. S6

Image

Figure Caption

Fig. S6 Additional Experiments, Related to Figure 7

(A) bdh2 is broadly expressed in zebrafish embryos. Whole-mount in situ hybridization analysis reveals that at 28 hr post fertilization (hpf) bdh2 mRNA is widely distributed throughout the zebrafish embryo. As a negative control, in situ hybridization was also performed using a bdh2 sense-strand probe (bottom).

(B) Confirmation of decreased hemoglobin levels in Bdh2-depleted zebrafish embryos using a second, unrelated bdh2 MO. (Top) Phenotypic analysis of control MO- and bdh2 MO-injected embryos at 72 hpf. (Bottom) Whole-mount o-dianisidine staining of control MO and bdh2 MO-injected embryos at 72 hpf. Shown are lateral (top) and ventral (bottom) views of the anterior region of embryos.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 141(6), Devireddy, L.R., Hart, D.O., Goetz, D.H., and Green, M.R., A mammalian siderophore synthesized by an enzyme with a bacterial homolog involved in enterobactin production, 1006-1017, Copyright (2010) with permission from Elsevier. Full text @ Cell