FIGURE

Fig. 5

ID

ZDB-FIG-100616-78

Publication

Znosko et al., 2010 - Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development

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Fig. 5

Pea3 ETS factors are required for heart development. (A?L) In situ hybridization showing cardiac gene expression at 24hpf (A?H) and 48hpf (I?L). Knock-down of multiple Pea3 ETS factors altered amhc (D; red arrowhead indicates midline of embryo) and vmhc expression (H; cell population and spatial arrangement in yellow bracket). Cardiac looping was severely affected in 3EtsMO-injected embryos as shown by expression of cmlc2 (K, L). (M) Quantification of effects on heart looping in MO-injected embryos as exemplified in I; normal looping, K; no looping, L; reverse looping.

Expression Data

Genes:	myh6 myh7 myl7
Fish:	AB AB + MO2-etv5a AB + MO2-etv5a + MO3-etv5a,etv5b + MO3-etv5b AB + MO2-etv5a + MO3-etv4 + MO3-etv5a,etv5b + MO3-etv5b
Knockdown Reagents:	MO2-etv5a MO3-etv4 MO3-etv5a,etv5b MO3-etv5b
Anatomical Terms:	heart presumptive atrium heart tube presumptive cardiac ventricle heart tube
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-etv5a + MO3-etv5a,etv5b + MO3-etv5b AB + MO2-etv5a + MO3-etv4 + MO3-etv5a,etv5b + MO3-etv5b
Knockdown Reagents:	MO2-etv5a MO3-etv4 MO3-etv5a,etv5b MO3-etv5b
Observed In:	determination of heart left/right asymmetry heart heart development heart looping presumptive atrium heart tube presumptive cardiac ventricle heart tube
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 342(1), Znosko, W.A., Yu, S., Thomas, K., Molina, G.A., Li, C., Tsang, W., Dawid, I.B., Moon, A.M., and Tsang, M., Overlapping functions of Pea3 ETS transcription factors in FGF signaling during zebrafish development, 11-25, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.