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Fig. 8 pigu is expressed ubiquitously by whole embryos. In situ hybridization with pigu antisense (A-J) or sense probes (K-T). Wholemounts and DIC images at the spinal cord showed that pigu is expressed by whole zebrafish embryos at 1 dpf (A,B) and 2 dpf (C,D). Expression of pigu mRNA was also observed at 0.2 hpf (1-cell stage; E), 0.75 hpf (2-cell stage; F), 1 hpf (4-cell stage; G), 1.25 hpf (8-cell stage; H), 4 hpf (sphere stage; I) and 11 hpf (early segmentation stage; J). Staining with sense probe displayed no pigu expression at any stages (K-T).
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