ZFIN ID: ZDB-FIG-100504-3
Grinblat et al., 2001 - zic gene expression marks anteroposterior pattern in the presumptive neurectoderm of the zebrafish gastrula. Developmental dynamics : an official publication of the American Association of Anatomists   222(4):688-693 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Observed In:
Stage: Shield

Fig. 4 Early pattern in the ectoderm, marked by zic3 expression, is regulated by bone morphogenetic protein (BMP) signaling. Embryos stained by whole-mount in situ hybridization (ISH) for expression of zic3 (orange in A, purple in C-F) and bmp2b/swr (purple in A). All embryos are shown in animal pole view, dorsal to the right. A: zic3 (orange) and bmp2b/swr (purple) are expressed in complementary domains in wild-type embryos. B: Diagram of a specification map, derived from in vitro explant culture assays, of the early gastrula ectoderm (Grinblat et al., [1998]). V, ventral non-neural ectoderm; A, anterior forebrain; P, posterior forebrain. C: Embryo derived from a din/+x din/+ cross, showing a wild-type pattern of zic3 expression. D: Sibling embryo from the same cross, showing a strong reduction in dorsal zic3 expression. Dorsal marginal staining remained unaffected (not shown). E: Embryo derived from a snh/+ x snh/+ cross, showing a wild-type pattern of zic3 expression. F: Sibling embryo from the same cross, showing up-regulation of zic3 expression in ventral posterior ectoderm (white asterisk). Note that the animal pole is still devoid of zic3 expression. d, dorsal; white asterisk marks ventral side.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
bmp2b WT standard conditions Shield presumptive ectoderm ISH
Shield presumptive forebrain ISH
zic3 WT standard conditions Shield presumptive ectoderm ISH
Shield presumptive forebrain ISH
bmp7aty68a/ty68a standard conditions Shield presumptive ectoderm ISH
Shield presumptive forebrain ISH
chrdtt250/tt250 standard conditions Shield Not Detected presumptive ectoderm ISH
Shield Not Detected presumptive forebrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bmp7aty68a/ty68a standard conditions Shield anterior/posterior pattern specification process quality, normal
Shield dorsal/ventral pattern formation disrupted, abnormal
chrdtt250/tt250 standard conditions Shield positive regulation of transcription, DNA-templated disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.