FIGURE

Fig. 9

ID

ZDB-FIG-100223-31

Publication

Camarata et al., 2010 - Pdlim7 (LMP4) regulation of Tbx5 specifies zebrafish heart atrio-ventricular boundary and valve formation

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Fig. 9

Pdlim7 and Tbx5 directly regulate tbx2b AV expression. (A-C) In situ hybridization of tbx2b 48 hpf at the AV boundary in wild-type (A), hst/+ (B), and hst/hst (C) embryos. (D) AV boundary expansion of tbx2b in wild-type embryo after 2 ng MO2 injection. (E) tbx2b expression in hst/+ embryo injected with MO2 or (F) injected with 75 pg pdlim7 mRNA. (G) Quantification of tbx2b expression levels in uninjected controls (n = 94), 2 ng MO2 (n = 70), and 75 pg mRNA (n = 92). (H-J) Schematics illustrating the effect on the Tbx5 target gene tbx2b in wild-type (H), Pdlim7 MO (I), or pdlim7 mRNA (J) injected embryos.

Expression Data

Gene:	tbx2b
Fish:	WT tbx5a^m21/m21 WT + MO4-pdlim7 tbx5a^m21/+ tbx5a^m21/+ + MO4-pdlim7
Knockdown Reagent:	MO4-pdlim7
Anatomical Term:	atrioventricular canal
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tbx5a^m21/m21 WT + MO4-pdlim7 tbx5a^m21/+ tbx5a^m21/+ + MO4-pdlim7
Knockdown Reagent:	MO4-pdlim7
Observed In:	atrial ventricular junction remodeling atrioventricular canal
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 337(2), Camarata, T., Krcmery, J., Snyder, D., Park, S., Topczewski, J., and Simon, H.G., Pdlim7 (LMP4) regulation of Tbx5 specifies zebrafish heart atrio-ventricular boundary and valve formation, 233-245, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.