Fig. 9 Pdlim7 and Tbx5 directly regulate tbx2b AV expression. (A-C) In situ hybridization of tbx2b 48 hpf at the AV boundary in wild-type (A), hst/+ (B), and hst/hst (C) embryos. (D) AV boundary expansion of tbx2b in wild-type embryo after 2 ng MO2 injection. (E) tbx2b expression in hst/+ embryo injected with MO2 or (F) injected with 75 pg pdlim7 mRNA. (G) Quantification of tbx2b expression levels in uninjected controls (n = 94), 2 ng MO2 (n = 70), and 75 pg mRNA (n = 92). (H-J) Schematics illustrating the effect on the Tbx5 target gene tbx2b in wild-type (H), Pdlim7 MO (I), or pdlim7 mRNA (J) injected embryos.
Reprinted from Developmental Biology, 337(2), Camarata, T., Krcmery, J., Snyder, D., Park, S., Topczewski, J., and Simon, H.G., Pdlim7 (LMP4) regulation of Tbx5 specifies zebrafish heart atrio-ventricular boundary and valve formation, 233-245, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.