Fig. 5

Analysis of zebrafish Neuroligins splicing pattern during embryo development and in adult organs. RT-PCR products were generated from total RNA extracted from different adult organs, oocytes, and stages of embryonic development. Specific beta actin primers (Argenton et al., [2004]) were used to check cDNA quality and possible genomic contamination. SS1 and SS2 indicate alternatively spliced sites of Neuroligin genes (Site A and Site B, respectively). Numbers on the left indicate the dimension in base pairs of the different molecular weights (MW). Control RT-PCR reactions without cDNA template were negative (data not shown).