ZFIN ID: ZDB-IMAGE-091215-62
Figures for Lee et al., 2009

Gene:

Figure Caption/Comments:

Fig. 1 Notch Signaling Negatively Regulates the Number of Endothelial Cells during Zebrafish Development

(A) Transverse sections of 18 hpf embryos, DMSO- or DAPT-treated, visualized for TOPRO (white), kdrl:GFP (green), and phalloidin (red).

(B) Quantification of endothelial nuclei per focal plane in DMSO-treated (n = 30) or DAPT-treated (n = 44) embryos.

(C) Transverse sections of 18 hpf phenotypic wild-type siblings and Tg(hsp70l:Gal4)kca4;Tg(UAS:myc-NICD)kca3 embryos visualized for TOPRO (white), kdrl:GFP (green), and c-Myc as a surrogate measure for NICD expression (red).

(D) Quantification of endothelial nuclei per focal plane in phenotypic wild-type (n = 31) and NICD-overexpressing (n = 28) embryos. Embryos treated with DAPT contained 6.66 (s = 2.42) endothelial nuclei per section, whereas those treated with DMSO had 4.03 (s = 1.27) endothelial nuclei (ANOVA, p < 10-6). Conversely, embryos overexpressing the NICD contained 3.14 (s = 0.80) endothelial nuclei, in comparison to 4.42 (s = 1.09) nuclei per section in phenotypic wild-type siblings (ANOVA, p < 10-5), suggesting that Notch signaling negatively regulates endothelial cell number.

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Acknowledgments:
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