Fig. 4

Reischauer et al., 2009 - Lgl2 executes its function as a tumor suppressor by regulating ErbB signaling in the zebrafish epidermis
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Fig. 4

Inhibition of ErbB signaling restores the epidermal morphology and cell cycle in pen/lgl2 mutant larvae.

Western blot analysis of Erk1/2 phosphorylation in untreated wild-type and pen/lgl2 larvae (A), larvae treated with PD168393 (B) and larvae treated with SU5402 (C). DIC images of wild-type (D) and pen/lgl2 mutant (E) treated with the ErbB inhibitor PD168393 and genotyped. The pan 1–8 cytokeratin antibody staining (F–H), anti E-cad staining (I–K), anti BrdU antibody staining (L–N) and in situ hybridization staining for mmp9 (O–Q) of the epidermis of wild-type (F, I, L, O) pen/lgl2 mutant (G, J, M, P) and mutant larvae treated with PD168393 (H, K, N, Q). In comparison to wild-type larvae (A, left lane, 44kD) Erk shows higher level of phosphorylation in pen/lgl2 mutant larvae (A, right lane 44kD). The levels of Erk phosphorylation are equal in the mutants treated with PD168393 (B) but not with SU5402 (C). The α-Tubulin levels (55kD) are indicative of equal protein loading. The epidermal cell morphology, E-cad localization, cell proliferation and mmp9 expression in pen/lgl2 larvae, treated with PD168393 (H, K, N, Q) appears similar to wild-type larvae (F, I, L, O) than the untreated mutant larvae (G, J, M, P). Note that PD168393 treated (rescued) larvae were genotyped to confirm their genotypes.

Expression Data
Anatomical Terms:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
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