Fig. S2

The embryos stained by hgg1/dlx3/ntl were at a comparable stage, and lats2 does not show any obvious relation to bmp4 in early development. A-C: The embryos defective in gastrulation movements were age-comparable to the wild-type embryos, as the embryos also showed normal labeling of krox20 at rhombomere 3 and 5 (B,C), which only appear from 3-somite stage. D-F: Expression of bmp4 at 4-somite stage in wild-type (D), lats1 morphant (E), and lats2 morphant (F) embryos. As the specific stronger expression region of lats2 during gastrulation is similar to that of bmp4, which suggests the possible involvement of lats2 activity with bmp4 pathway. However, the expression of bmp4 in the four-somite stage lats1/lats2 morphants was nearly unchanged when compared with that in wild-type embryos. G-I: In accordance with this, at 75%-epiboly stage, ventral mesoderm specific eve1 labeling and the dorsal anterior mesoderm specific gsc labeling did not show any significant difference between the wild-type embryos and the lats1/2 morphants. These indicate that there may be a very weak, if any, relation between lats1/2 and bmp4 in the early development of zebrafish. Embryos in A-C are dorsal view with anterior to the top, and embryos in D-I are lateral view with dorsal to the right.