PUBLICATION

Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements

Authors

Chen, C.H., Sun, Y.H., Pei, D.S., and Zhu, Z.Y.

ID

ZDB-PUB-091023-62

Date

2009

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 238(11): 2850-2859 (Journal)

Registered Authors

Pei, Desheng, Sun, Yonghua, Zhu, Zuoyan

Keywords

zebrafish, lats, phylogenetic analysis, spatiotemporal expression, covergence extention movement

MeSH Terms

Phylogeny
Animals
Cloning, Molecular
Gastrula/drug effects
Gastrula/embryology*
Gastrula/enzymology
Protein Serine-Threonine Kinases/biosynthesis*
Protein Serine-Threonine Kinases/genetics
Embryo, Nonmammalian/embryology*
Embryo, Nonmammalian/enzymology
Gastrulation*
Oligonucleotides, Antisense/genetics
Zebrafish/embryology*
Zebrafish/metabolism

(all 14)

PubMed

19842174 Full text @ Dev. Dyn.

Abstract

Large tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In zebrafish, both lats genes are maternally expressed, while they show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula. Most intriguingly, as revealed by cell tracing and gene expression analysis, morpholino-mediated knockdown of either lats1 or lats2 led to obvious defects of cell migration in gastrulation, indicating the functional significance of lats in gastrulation movements.

Genes / Markers

Figures

Show all Figures

Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
AB	standard conditions	Adult	brain	RTPCR	Fig. 2
AB	standard conditions	Long-pec	brain	ISH	Fig. 2
AB	standard conditions	Adult	eye	RTPCR	Fig. 2
AB	standard conditions	Prim-5	head	ISH	Fig. 2
AB	standard conditions	Adult	heart	RTPCR	Fig. 2
AB	standard conditions	Adult	kidney	RTPCR	Fig. 2
AB	standard conditions	Adult	liver	RTPCR	Fig. 2
AB	standard conditions	Adult	muscle	RTPCR	Fig. 2
AB	standard conditions	Adult	oocyte	RTPCR	Fig. 2
AB	standard conditions	Long-pec	pectoral fin	ISH	Fig. 2

1 - 10 of 63

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
AB + MO1-lats1	standard conditions	1-4 somites	convergent extension involved in gastrulation disrupted, abnormal	text only
AB + MO1-lats2	standard conditions	1-4 somites	convergent extension involved in gastrulation disrupted, abnormal	text only
AB + MO2-lats1	standard conditions	1-4 somites	apoptotic process occurrence, normal	Fig. 4
AB + MO2-lats1	standard conditions	1-4 somites	convergent extension involved in gastrulation disrupted, abnormal	Fig. 3
AB + MO2-lats1	standard conditions	Bud	convergent extension involved in gastrulation disrupted, abnormal	Fig. 3
AB + MO2-lats1 + MO2-lats2	standard conditions	Bud to 1-4 somites	convergent extension involved in gastrulation disrupted, abnormal	Fig. S1
AB + MO2-lats2	standard conditions	1-4 somites	apoptotic process occurrence, normal	Fig. 4
AB + MO2-lats2	standard conditions	1-4 somites	convergent extension involved in gastrulation disrupted, abnormal	Fig. 3
AB + MO2-lats2	standard conditions	Bud	convergent extension involved in gastrulation disrupted, abnormal	Fig. 3

1 - 9 of 9

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
lats1	MO1-lats1	MRPHLNO
lats1	MO2-lats1	MRPHLNO
lats2	MO1-lats2	MRPHLNO
lats2	MO2-lats2	MRPHLNO

Fish

Fish
AB
AB + MO1-lats1
AB + MO1-lats2
AB + MO2-lats1
AB + MO2-lats1 + MO2-lats2
AB + MO2-lats2

Antibodies

Orthology

Gene	Orthology
lats1
lats2

Engineered Foreign Genes

Mapping

Chen et al., 2009 ZDB-PUB-091023-62 PMID:19842174

Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements

Chen et al., 2009

ZDB-PUB-091023-62
PMID:19842174