PUBLICATION

Comparative expression of zebrafish lats1 and lats2 and their implication in gastrulation movements

Authors
Chen, C.H., Sun, Y.H., Pei, D.S., and Zhu, Z.Y.
ID
ZDB-PUB-091023-62
Date
2009
Source
Developmental dynamics : an official publication of the American Association of Anatomists   238(11): 2850-2859 (Journal)
Registered Authors
Pei, Desheng, Sun, Yonghua, Zhu, Zuoyan
Keywords
zebrafish, lats, phylogenetic analysis, spatiotemporal expression, covergence extention movement
MeSH Terms
  • Animals
  • Cloning, Molecular
  • Embryo, Nonmammalian/embryology*
  • Embryo, Nonmammalian/enzymology
  • Gastrula/drug effects
  • Gastrula/embryology*
  • Gastrula/enzymology
  • Gastrulation*
  • Oligonucleotides, Antisense/genetics
  • Phylogeny
  • Protein Serine-Threonine Kinases/biosynthesis*
  • Protein Serine-Threonine Kinases/genetics
  • Zebrafish/embryology*
  • Zebrafish/metabolism
PubMed
19842174 Full text @ Dev. Dyn.
Abstract
Large tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In zebrafish, both lats genes are maternally expressed, while they show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula. Most intriguingly, as revealed by cell tracing and gene expression analysis, morpholino-mediated knockdown of either lats1 or lats2 led to obvious defects of cell migration in gastrulation, indicating the functional significance of lats in gastrulation movements.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping