Fig. 2

Higher magnification of the gene expression of zebrafish fzd7a, 7b and 8a reveals specific patterning within the head. (A–I) Lateral views of the embryo show all three genes expressed within the pharyngeal arches (arrowheads). (A–C) Gene expression of fzd7a, 7b and 8a at 2 days post-fertilization (dpf). (A) fzd7a is expressed in the dorsal part of the hindbrain (arrow) and within the forebrain (asterisk). (B,C) fzd7b and 8a are expressed within the anterior lateral line (asterisks). (D–F) Gene expression of fzd7a, 7b and 8a at 3 dpf. (D) fzd7a is clearly expressed in the tectum (arrow). (E,F) fzd7b and 8a are expressed within the anterior lateral line (asterisks). (G–I) Gene expression of fzd7a, 7b and 8a at 4 dpf. (G) The gene expression of fzd7a in the dorsal hindbrain is still visible. (J–L) Dorsal views of fzd7a, 7b and 8a gene expression at 2 dpf. (J) A superficial dorsal view of 2 dpf clearly shows the tectum (arrow), rhombic lip (asterisk) and dorsal rhombomere expression (arrowhead) of fzd7a. fzd7b and 8a are expressed at the midline (arrowhead) and in the otic vesicle (arrow) (K and L, respectively). (M–O) Dorsal views of the gene expression of fzd7a, 7b and 8a at 3 dpf. (M) The expression in the in the rhombic lip and dorsal rhombomeres (arrowhead) has moved laterally. (O) The dorsal view of fzd8a expression clearly shows the anterior lateral line expression (asterisks). (P–R) Ventral views of fzd7a, 7b and 8a gene expression at 3 dpf. (P) fzd7a is expressed in the retina adjacent to the lens (arrow). (Q–R) Ventral views of fzd7a and 7b expression reveal expression in the anterior lateral line (asterisk). (R) At 3 dpf fzd8a is expressed within the olfactory bulbs (arrows). Bar equals 100 μm.