FIGURE

Fig. 10

ID

ZDB-FIG-090710-60

Publication

Yabe et al., 2009 - The maternal-effect gene cellular island encodes aurora B kinase and is essential for furrow formation in the early zebrafish embryo

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Fig. 10

Redundant signals contribute to furrow formation in the early blastomeres.

Wild-type (A–D) and cei; fue double mutant (E–H) embryos were fixed at 49 min p.f. and labeled with anti–α-tubulin antibodies (A,E; red in D,H), anti–γ-tubulin antibodies (B,F; green in D,H) and DAPI (C,G; blue in D,H). (A–D) At this stage, wild-type embryos exhibit an FMA along the span of the first cleavage plane (1^st). (E–H) cei; fue double mutant embryos lack an FMA in either medial or distal regions of the 1^st cleavage plane (compare to Figure 3D,H). Double mutants show the expected delay in furrow induction, characteristic of the cei mutation, in the less mature furrow corresponding to the 2^nd cleavage plane (arrowhead in (E); compare to Figure 3B and 3F). Double mutants also exhibit the pronuclear fusion defect characteristic of the fue mutation [31]. Similar results can be observed in embryos until 65 min p.f. (not shown).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lrmp^t1497/t1497; aurkb^p63cd/p63cd
Observed In:	blastoderm cleavage furrow
Stage:	2-cell

Phenotype Detail

Acknowledgments

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