FIGURE

Fig. 5

ID

ZDB-FIG-090710-55

Publication

Yabe et al., 2009 - The maternal-effect gene cellular island encodes aurora B kinase and is essential for furrow formation in the early zebrafish embryo

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Fig. 5

Maternal expression of wild-type aurora B kinase rescues the cytokinesis defects in cei mutant embryos.

Embryos from cei/cei homozygous mutant females show a strong syncytial phenotype at the 1,000 cells stage (A) and show defective accumulation of β-catenin in mature furrows at the 8-cell stage (C). Embryos from sibling cei/cei homozygous mutant females which carry transgenic copies of a maternally-expressed wild-type zebrafish aurB gene show significant rescue of the syncytial (B) and β-catenin furrow accumulation phenotype (D). Side views of 1,000-cell stage live embryos (A,B) and animal views of embryos fixed at the 8-cell stage and labeled with an anti-β-catenin antibody (green) and a DNA dye (blue) (C,D). Homozygosity for cei and presence of the transgene were determined by genotyping as in Materials and Methods.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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