FIGURE

Fig. 8

ID

ZDB-FIG-090710-39

Publication

Lunt et al., 2009 - Zebrafish ift57, ift88, and ift172 intraflagellar transport mutants disrupt cilia but do not affect hedgehog signaling

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Fig. 8

A low dose of cyclopamine does not affect Hh signaling in IFT mutants. A: Expression of ptc-1 is unaffected in 24-hpf embryos following addition of 5 μM cyclopamine to progeny of IFT heterozygous incrosses. At least 30 embryos were examined from each incross. Two representative embryos from each clutch are shown. Images show a lateral view of embryos, with anterior to the left. B: In situ hybridization experiment demonstrating that ptc-1 expression is increased in lep mutants. Arrow shows increased ptc-1 in the forebrain of lep mutants. High magnification reveals increased ptc-1 expression in the trunk of lep1 mutants (middle panel). Quantification of phenotypes are shown in the graph. C: At 5 dpf, the lens degeneration in lep mutants can be observed. The graph shows that addition of cyclopamine rescues this phenotype.

Expression Data

Gene:	ptch2
Fish:	WT ift57^{hi3417Tg/hi3417Tg} ift88^tz288/tz288 ift172^{hi2211Tg/hi2211Tg} ptch1^tj222/tj222
Condition:	chemical treatment: Cyclopamine
Anatomical Terms:	forebrain neural tube somite
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ptch1^tj222/tj222
Condition:	chemical treatment: Cyclopamine
Observed In:	lens
Stage:	Day 5

Phenotype Detail

Acknowledgments

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