Alcian blue staining of jaw and brachial arch structure at 5 dpf. A-C: Wild type embryos raised in DMSO vehicle exhibit normal craniofacial head skeleton. D-L: IFT mutants displayed no reduction or loss of the major head structures and no fusion of the trabeculae. M-O: detourgli1 mutants showed significant narrowing of ethmoid plate (black arrow). P: Embryos treated with 100 μM cyclopamine lack all major head cartilage. The top row shows whole-mount images viewed from the ventral side. The middle row shows flat-mount images of dissected dorsal neurocranium structures. The bottom row shows flat-mounted images of dissected ventral structures to show brachial arches.
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