FIGURE

Fig. 3

ID

ZDB-FIG-090602-10

Publication

Rothschild et al., 2009 - Tbx5-mediated expression of Ca(2+)/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin morphogenesis

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Fig. 3

Tbx5 heart defects can be rescued by CaMK-II. Wild type or cmlc2:DsRed transgenic embryos (k?o) were injected with the mismatch MO (5 ng), camk2b2 MO (2 ng) or tbx5 MO (5 ng) with or without 40 pg wild type δ_C CaMK-II cDNA and imaged at 72 hpf (a?e; k?y) or subjected to cmlc2 in situ hybridization at 48 hpf (f?j). Scale bar = 0.5 mm and anterior is to the left. In f and k, a = atrium and v = ventricle. In p?t, insets reveal bmp4 in situ hybridization patterns in the pectoral fin area. In u?y, brackets indicate extent of pectoral fin length.

Expression Data

Genes:	bmp4 DsRed2 myl7
Fish:	WT WT + MO2-camk2b2 WT + MO2-tbx5a vc6Tg vc6Tg + MO2-camk2b2 vc6Tg + MO2-tbx5a (all 6)
Knockdown Reagents:	MO2-camk2b2 MO2-tbx5a
Anatomical Terms:	heart pectoral fin
Stage Range:	Long-pec to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-camk2b2 WT + MO2-tbx5a
Knockdown Reagents:	MO2-camk2b2 MO2-tbx5a
Observed In:	heart heart looping pectoral fin
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 330(1), Rothschild, S.C., Easley, C.A. 4th, Francescatto, L., Lister, J.A., Garrity, D.M., and Tombes, R.M., Tbx5-mediated expression of Ca(2+)/calmodulin-dependent protein kinase II is necessary for zebrafish cardiac and pectoral fin morphogenesis, 175-184, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.