Fig. 5

Fig. 5 Immunohistochemical analysis of wild type, ift57, ift88, and ift172 mutant zebrafish at 60 hpf. (A–D) 1D1 (green), a marker for rhodopsin, localized to the outer segment region in wild type and ift57 mutants. Strong mislocalization to the inner segment was also observed in ift57 mutants, as well as ift88, and ift172 mutants. (E–H) Zpr1 (green), a label for red/green double cones gave an elongated, columnar morphology in wild type animals. Cone morphology was disheveled in the three mutants. (I–J) Blue opsin (BOPS) was seen in small foci of wild type photoreceptors. Similar staining was seen in ift57, ift88, and ift172 mutants. (M–P) IFT52 (red) co-localized with acetylated tubulin (green) in the connecting cilia of wild type animals (arrows). No such staining was observed in ift57, ift88, and ift172 mutants. (Q–T) IFT88 (red) also showed apical localization in wild type animals (arrows). The staining partially overlapped with acetylated tubulin (green). No such staining was observed in IFT mutants. (U–X) The basal body marker centrin (red) localized to the apical surface of the inner segment. Acetylated tubulin (green) denotes microtubules. Centrin was seen in wild type and all IFT mutants (arrows). In all images, the tissues were counterstained with DAPI (blue). Scale bar = 20 μm.