FIGURE

Fig. 6

ID

ZDB-FIG-090224-51

Publication

Dowling et al., 2009 - Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathy

Other Figures

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Fig. 6

Increased PI3P levels in myotubularin morphant myofibers.

(A) Representative myofibers immunostained with anti-PI3P. Perinuclear staining of PI3P in myotubularin morphant myofibers is much more abundant than in control myofibers (arrows). There is also a modest increase in membrane localized PI3P (*). Scale bar = 10 mm. (B) Quantitation of PI3P immunofluorescence. PI3P intensity measured over a uniform perinuclear area (see methods for details) and was 83.7+/-7.8 pixels for control morphants and 135.5+/-3.3 for myotubularin morphants (3 trials; p = 0.0027). This represented a 1.6x increase in PI3P staining intensity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-mtm1
Knockdown Reagent:	MO1-mtm1
Observed In:	phosphatidylinositol dephosphorylation
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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