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General Information
ZFIN ID: ZDB-FIG-090224-52
Dowling et al., 2009 - Loss of myotubularin function results in T-tubule disorganization in zebrafish and human myotubular myopathy. PLoS Genetics   5(2):e1000372 Full text @ PLoS Genet.
Anatomical Term:
Stage: Prim-5

Fig. 7 MTMR rescue of the myotubularin morphant phenotype.

(A) Whole mount in situ hybridization of 24 hpf embryos reveals muscle staining for myotubularin (MTM1 AS) and not for MTMR1 or MTMR2 (data not shown). A sense probe to MTM1 (MTM1 S) was used as a background control. (B) RNA rescue experiment. RNA to MTM1, MTMR1 or MTMR2 was co-injected with myotubularin morpholino. Rescue was determined by the % of hatched embryos at 60 hpf. Values were: No RNA (35.5%+/-3.3%, N = 201), MTM1 RNA (71%+/-4.5, N = 100, p (when compared to No RNA)<0.0001), MTMR1 RNA (82%+/-5.5%, N = 50, p<0.001), MTMR2 RNA (54.6%+/-5.1% N = 97, p = 00015).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mtm1 WT standard conditions Prim-5 muscle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.