header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-090223-9
Vasilyev et al., 2009 - Collective Cell Migration Drives Morphogenesis of the Kidney Nephron. PLoS Biology   7(1):e9 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 8 Eliminating Glomerular Filtration Results in Ectopic Tubule Convolution

All embryos were imaged at 84 hpf.

(A and B) Control condition, ET33-D10 transgenics (A) and ET11–9 transgenics (B).

(C and D) Cardiac tnnt2 morpholino injected embryos, ET33-D10 transgenic (C) and ET11–9 transgenic (D).

(E and F) Cardiac tnnt2 and ift88 (polaris) morpholino injected embryos, ET33d10 transgenic (E) and ET11–9 transgenic (F).

(G–M) Anti-alpha6F NaK ATPase stained embryos. (G) Control embryo. (H and I) Close-ups of the proximal and mid segments. (J) Cardiac tnnt2 morpholino injected embryo. (K and L) Close-ups of the proximal and the mid segments. (M) Cardiac tnnt2 and ift88(polaris) morpholino-injected embryo.

(N) ET11–9 GFP transgenic embryo injected with cardiac tnnt2 morpholino, stained with anti-alpha6 NaK ATPase (faint), and anti-GFP (bright).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP sqet11Et standard conditions Protruding-mouth pronephros IFL
sqet11Et + MO1-tnnt2a standard conditions Protruding-mouth pronephros IFL
sqet11Et + MO1-tnnt2a + MO2-ift88 standard conditions Protruding-mouth pronephros IFL
sqet33D10Et(AB) standard conditions Protruding-mouth pronephros IFL
sqet33D10Et + MO1-tnnt2a(AB) standard conditions Protruding-mouth pronephros IFL
sqet33D10Et + MO1-tnnt2a + MO2-ift88(AB) standard conditions Protruding-mouth pronephros IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sqet11Et + MO1-tnnt2a standard conditions Protruding-mouth distal tubule development arrested, abnormal
Protruding-mouth proximal convoluted tubule development arrested, abnormal
Protruding-mouth simple columnar epithelium displaced, abnormal
Protruding-mouth simple cuboidal epithelium displaced, abnormal
sqet11Et + MO1-tnnt2a + MO2-ift88 standard conditions Protruding-mouth distal tubule development process quality, normal
Protruding-mouth proximal convoluted tubule development process quality, normal
sqet33D10Et + MO1-tnnt2a(AB) standard conditions Protruding-mouth distal tubule development arrested, abnormal
Protruding-mouth proximal convoluted tubule development arrested, abnormal
Protruding-mouth simple columnar epithelium displaced, abnormal
Protruding-mouth simple cuboidal epithelium displaced, abnormal
sqet33D10Et + MO1-tnnt2a + MO2-ift88(AB) standard conditions Protruding-mouth distal tubule development process quality, normal
Protruding-mouth proximal convoluted tubule development process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.