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Fig. 1 Definitive erythroid cells arise in the PBI but not the VDA. (A-K) Whole-mount in situ hybridization to detect βe1-globin expression in wild-type embryos (A-E) at 22 hpf (A), 2 dpf (B), 3 dpf (C), 4 dpf (D) and 5 dpf (E); runx1w84x mutant (F,G) and morphant (H) embryos at 22 hpf (F) and 4 dpf (G,H); and vltm651 embryos (I-K) at 22 hpf (I), 2 dpf (J) and 4 dpf (K). The blue arrow in E indicates βe1-globin expression in pronephros. The insets are higher magnification (20x) views of the corresponding boxed regions (blue).
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