Fig. 7

Zebrafish V2b cells are VeLDs and ventral gata/scl-expressing cells are KAs. Lateral views of the spinal cord of Tg(8.1kGata1:eGFP) embryos (Kobayashi et al., 2001) at 24–25 h. Immunohistochemistry for GFP (green) and in situ hybridisation for gata3 (red; A and C), scl (red; B and D) or GABAergic markers (red; E–G). Single channel views and merged images are shown for panels A–D. Panels A and B are projections of multiple optical sections at the focal levels at which V2 cells/VeLDs are found. Panels C and D are projections of multiple optical sections at the focal levels at which KA cells are found. Merged images are on top followed by green channel and then red channel images. Panels E–G are projections of multiple optical sections that include the focal levels at which V2 cells/VeLDs and KA cells are found. Panel F is a higher magnification view of the left hand white dotted box in panel E indicating the morphology of 2 VeLDs and panel G is a higher magnification view of the right hand white dotted box in panel E indicating the morphology of a KA cell. Example cell morphologies are indicated by a star in the cell soma and arrow heads pointing to the axon trajectory of that cell. All of the GFP-positive cells express gata3, scl and GABAergic markers. Anterior is left, dorsal is up. Scale bar = 50 μm (A–E).