Fig. 2
Uninfected Cells at WT Granulomas Show Distinct Morphology and Rapid Motility and Infection (A–C) Hoechst-positive nuclei (blue) are distinguishable as uninfected (A) or infected (B). White arrows, Hoechst-positive nuclei; arrowheads, bacteria (green). (C) Infected Hoechst-positive cells in WT versus ΔRD1 lesions over 24 hr. p from Mann-Whitney test. (D and E) Distinct morphologies of uninfected cells at WT (D) and ΔRD1 (E) lesions. (D) Left: highly motile cell at WT granuloma with lamellipodium (white bracket), elongated nucleus (black bracket), and uropod (large black arrowhead). Arrow indicates direction of travel. Right: distinct appearance in WT granulomas of highly vesicular macrophage shortly before phagocytosis. Small black arrowheads, vesicles. Scale bars, 5 μm. (E) Less motile cell at ΔRD1 granuloma with no lamellipodium and rounded nucleus (black bracket). Scale bar, 5 μm. (F) Comparison of overall cell length, left, and nuclear aspect ratio, right, of uninfected cells at WT and ΔRD1 granulomas. p by unpaired Student′s t test. (G) Speeds of uninfected cells at WT or ΔRD1 granulomas, or at the site of injection 1 hr post-infection in the hindbrain ventricle. Bracket above indicates results of 1 way ANOVA (Kruskal-Wallis test)—uninfected cells at ΔRD1 granulomas (***) differed significantly from all others (p < 0.005), other differences not significant. (H–K) Tracks of uninfected cells in (G). (H and I) Cells at WT and ΔRD1 granulomas—all cells tracked for each strain from one granuloma. Insets, fluorescence view of whole granuloma. Inset scale bar, 30 μm. (J and K) Cells at 1 hr post-injection. 1 hpi tracks from two WT-infected embryos and three ΔRD1-infected embryos. All scale bars = 5 μm unless noted otherwise. |
Reprinted from Cell, 136(1), Davis, J.M., and Ramakrishnan, L., The role of the granuloma in expansion and dissemination of early tuberculous infection, 37-49, Copyright (2009) with permission from Elsevier. Full text @ Cell