FIGURE

Fig. 1

ID

ZDB-FIG-090127-52

Publication

Regan et al., 2009 - An Fgf8-dependent bistable cell migratory event establishes CNS asymmetry

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Fig. 1

The fgf8 Mutant Has a Symmetric Epithalamus

(A–D) Dorsal views of confocal images of the epithalamus in wild-type and ace 3 dpf embryos, with anterior to the top. (A and B) 3D reconstructions of pineal/parapineal nuclei and axons [green, Tg(foxD3:GFP)] and neuropil of the habenular nuclei (red, anti-acetylated tubulin; white-edged arrowheads). (C and D) The parapineal-specific marker Tg(ET11:GFP) is present (green), but expressing cells remain at the midline in the ace embryo compared with those of the wild-type embryo at 3 dpf. Brain morphology is visualized using the nuclear marker TOPRO3 (red). A single z-slice is presented for each example. (E and F) Frontal view of parapineal-specific gfi expression in wild-type and ace 3 dpf embryos, with dorsal to the top. Parapineal cells are at the midline in ace (F). (G–J) Dorsal views of habenular lov (G and H) and brn3a (I and J) expression in wild-type and ace embryos at 4 dpf; expression of both markers is reduced in left and right habenulae.

Expression Data

Genes:	EGFP gfi1ab GFP kctd12.1 pou4f1
Antibody:	Ab1-tuba
Fish:	AB/TU fgf8a^{ti282a/ti282a} sqet11Et zf15Tg fgf8a^{ti282a/ti282a}; sqet11Et fgf8a^{ti282a/ti282a}; zf15Tg
Anatomical Terms:	habenula parapineal organ pineal complex
Stage Range:	Protruding-mouth to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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