PUBLICATION
An Fgf8-dependent bistable cell migratory event establishes CNS asymmetry
- Authors
- Regan, J.C., Concha, M.L., Roussigne, M., Russell, C., and Wilson, S.W.
- ID
- ZDB-PUB-090123-4
- Date
- 2009
- Source
- Neuron 61(1): 27-34 (Journal)
- Registered Authors
- Concha, Miguel, Regan, Jenny, Russell, Claire, Wilson, Steve
- Keywords
- DEVBIO, MOLNEURO, SIGNALING
- MeSH Terms
-
- Body Patterning/physiology
- Zebrafish/anatomy & histology*
- Zebrafish/embryology*
- Zebrafish/metabolism
- Nodal Protein/genetics
- Nodal Protein/metabolism
- Oligonucleotides, Antisense/genetics
- Oligonucleotides, Antisense/metabolism
- Forkhead Transcription Factors/genetics
- Forkhead Transcription Factors/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Cell Movement/physiology*
- Central Nervous System/anatomy & histology*
- Central Nervous System/embryology*
- Central Nervous System/metabolism
- Animals
- Signal Transduction/physiology
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/metabolism*
- PubMed
- 19146810 Full text @ Neuron
Citation
Regan, J.C., Concha, M.L., Roussigne, M., Russell, C., and Wilson, S.W. (2009) An Fgf8-dependent bistable cell migratory event establishes CNS asymmetry. Neuron. 61(1):27-34.
Abstract
Neuroanatomical and functional asymmetries are universal features of the vertebrate CNS, but how asymmetry is generated is unknown. Here we show that zebrafish fgf8 mutants do not elaborate forebrain asymmetries, demonstrated by the failure of the parapineal nucleus to migrate from its initial midline position to the left side of the brain. Local provision of Fgf8 restores the asymmetric migration of parapineal cells, usually to the left, irrespective of the location of the Fgf8 source. This laterality bias is due to left-sided Nodal signaling and when the bias in Nodal signaling is removed, parapineal cells migrate toward the source of Fgf8 protein. This study presents a mechanism for breaking neuroanatomical symmetry through Fgf8-dependent regulation of bistable left- or right-sided migration of the parapineal. The combined action of Fgf and Nodal signals ensures the establishment of neuroanatomical asymmetries with consistent laterality.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping