FIGURE

Fig. 4

ID

ZDB-FIG-090127-35

Publication

Sittaramane et al., 2009 - The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish

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Fig. 4

Neuronal defects in tag1 morphants. Panels A, B, E–J show dorsal views of the head with anterior to the left (anterior at top in G, H). Panels C, D, K and L show lateral views of the head with anterior to the left. tag1 morphants (D, F, and H) were co-injected with p53 MO to minimize non-specific apoptosis (Robu et al., 2007). WT embryos (controls) either uninjected or injected with p53 MO alone exhibited identical phenotypes in the tissues examined. (A, B) Migration of FBMNs (arrowheads) into r6 and r7 (A) is mostly eliminated in a tag1 MO-injected embryo (B). Zn5-labeled dorsal commissural neurons (red) at rhombomere boundaries develop in similar numbers in control and morphant embryos. (C, D) While acridine orange (AO) labeling reveals very few dying cells in a control embryo (C), there are many more dying cells (arrowhead) in a tag1 morphant (D). Asterisk marks otic vesicle. (E) In a control embryo, FBMNs migrate in close proximity to the medial longitudinal fascicle (MLF, arrowhead). Arrow indicates the dorsal longitudinal fascicle (DLF) containing the central (afferent) axons of the trigeminal sensory neurons. (F) In a tag1 morphant, the MLF (arrowhead) and DLF (arrow) appear brighter, but develop normally. (G, H) The trigeminal sensory ganglion (arrowhead) in a control embryo (G) is compact (inset shows lateral view), with prominent peripheral axons (arrowhead). In contrast, the trigeminal ganglion in a tag1 morphant (H) is composed of loosely organized cells (arrowheads, and inset showing lateral view), with peripheral axons (arrow) extending broadly over the head. Asterisks indicate the nucleus of the MLF, with brightly-labeled projections in the morphant, compared to the control embryo. (I, J) Expression of krox20 in r3 and r5 is similar between control and tag1 morphant embryos. (K, L) Ventrocaudal migration of tyrosine hydroxylase (TH)-positive locus coeruleus neurons (arrowhead) into r1 is similar between control and tag1 morphant embryos. Scale bar in D (150 μm for C, D); in E (75 μm for A, B, E–H); in J (75 μm for I–L).

Expression Data

Genes:	egr2b GFP th
Antibodies:	Ab1-tuba zn-5
Fish:	WT WT + MO1-cntn2 rw0Tg rw0Tg + MO1-cntn2
Knockdown Reagent:	MO1-cntn2
Anatomical Terms:	brainstem and spinal white matter facial nerve motor nucleus motor neuron lateral longitudinal fasciculus locus coeruleus medial longitudinal fasciculus rhombomere 3 rhombomere 5 trigeminal ganglion
Stage Range:	20-25 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-cntn2 rw0Tg + MO1-cntn2
Knockdown Reagent:	MO1-cntn2
Observed In:	facial nerve motor nucleus motor neuron locus coeruleus neuron migration trigeminal ganglion
Stage Range:	Prim-5 to Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 325(2), Sittaramane, V., Sawant, A., Wolman, M.A., Maves, L., Halloran, M.C., and Chandrasekhar, A., The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish, 363-373, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.