ZFIN ID: ZDB-FIG-081202-1
Rodriguez et al., 2008 - The receptor protein-tyrosine phosphatase, Dep1, acts in arterial/venous cell fate decisions in zebrafish development. Developmental Biology   324(1):122-130 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 4-cell to Long-pec

Fig. 1 Dep1a and Dep1b are orthologs of human Dep1 and are broadly expressed during early development. (A) Phylogenetic tree of Dep1 and its most closely related family members, PTPβ and SAP1. The PTP domains of zebrafish (D. rerio), human (H. sapiens) and mouse (M. mus) R3 subfamily members were plotted, based on sequence identity. (B) Genomic localization of human Dep1 (Chr. 11), Dep1a (LG7) and Dep1b (LG25) and genes in their immediate surroundings. (C–L) Dep1a- and dep1b-specific riboprobes, encompassing the catalytic domain, were generated and used in whole-mount in situ hybridization. Whole-mount expression of dep1a (C–E) and dep1b (F–H) during early zebrafish development, 4 cell stage (C,F), 4 hpf (D,G) and 30 hpf (E,H) are depicted as well as sections of 30 hpf and 48 hpf embryos (I–L). Relevant structures were labeled: DA, dorsal aorta; NC, notochord, NT, neural tube; PCV, posterior cardinal vein.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ptprja AB/TL standard conditions 4-cell whole organism ISH
Sphere whole organism ISH
Prim-15 blood vessel endothelial cell ISH
Prim-15 brain ISH
Prim-15 muscle ISH
Long-pec blood vessel endothelial cell ISH
Long-pec brain ISH
Long-pec muscle ISH
ptprjb.1 AB/TL standard conditions 4-cell whole organism ISH
Sphere whole organism ISH
Prim-15 blood vessel endothelial cell ISH
Prim-15 brain ISH
Prim-15 muscle ISH
Long-pec blood vessel endothelial cell ISH
Long-pec brain ISH
Long-pec muscle ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 324(1), Rodriguez, F., Vacaru, A., Overvoorde, J., and den Hertog, J., The receptor protein-tyrosine phosphatase, Dep1, acts in arterial/venous cell fate decisions in zebrafish development, 122-130, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.