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ZFIN ID: ZDB-FIG-081117-47
Bessarab et al., 2008 - Six1a is required for the onset of fast muscle differentiation in zebrafish. Developmental Biology   323(2):216-228 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to High-pec

Fig. 7 Muscle phenotype of six1a morphants is associated with abnormal differentiation of fast fibers. (A–C) Lateral view of embryos at 24 hpf, (left) and high magnification of boxed regions of the trunk (right). U-shaped somites in the embryos injected with UM or SM (B, C). UM (95/100 injected embryos); SM (91/100 injected embryos). (D–F) Lateral view of embryos at 42 h. Composite bright-field and polarized light (dark-field) images of trunk muscles. Original polarized light images are shown on the right. Note the reduction of muscle birefringence in the embryos injected with UM (E) and SM (F). (G–H) Confocal saggital sections of the trunk muscle at the level of anterior somites 7–9 in control embryos (left) and morphants (right). (G, G′) Immunostaining with the cocktail of mAb A4.1025 (anti-MyHC, red), ab6301 (anti-β-Catenin, green) and DAPI (nuclei, blue). Boxed region of panel G is enlarged in panel G′. Note that the diagonal array of cross-striated fast fibers (red) was not formed and regularity of cellular shapes (green) is absent in morphants. Arrowheads point to the regions with closely positioned nuclei. (H) Staining with F310 (anti-fast MyLC, red). Note the irregular shapes of cells and absence of arrayed organization in morphant fast muscle. (I) Confocal projection of superficial region of myotome after immunostaining with F59 (anti-slow MyHC, red). Note that slow fibers retained their normal position and morphology. (J) Confocal projection of medial region of myotome after immunostaining with 4D9 (anti-Engrailed, red). Scale bars, 300 μm in panels A–C, left; 70 μm in panels A–C, right; 100 μm in panels D–F; 30 μm in panel panel G; 15 μm in panel G′; 30 μm in panels H, I; 50 μm in panel J.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-A4.1025 IHC AB standard conditions Prim-5 fast muscle cell myofibril
IHC Prim-5 trunk musculature
IHC AB + MO4-six1b standard conditions Prim-5 fast muscle cell myofibril
IHC Prim-5 trunk musculature
Ab-F59 IHC AB standard conditions Prim-5 myotome
IHC Prim-5 slow muscle cell myofibril
IHC Prim-5 trunk musculature
IHC AB + MO4-six1b standard conditions Prim-5 myotome
IHC Prim-5 slow muscle cell myofibril
IHC Prim-5 trunk musculature
Ab-F310 IHC AB standard conditions Prim-5 fast muscle cell myofibril
IHC Prim-5 trunk musculature
IHC AB + MO4-six1b standard conditions Prim-5 fast muscle cell myofibril
IHC Prim-5 trunk musculature
Ab1-ctnnb IHC AB standard conditions Prim-5 skeletal muscle cell
IHC Prim-5 trunk musculature
IHC AB + MO4-six1b standard conditions Prim-5 skeletal muscle cell
IHC Prim-5 trunk musculature
Ab1-eng IHC AB standard conditions Prim-5 muscle pioneer
IHC Prim-5 myotome
IHC Prim-5 trunk musculature
IHC AB + MO4-six1b standard conditions Prim-5 muscle pioneer
IHC Prim-5 myotome
IHC Prim-5 trunk musculature
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO4-six1b standard conditions Prim-5 fast muscle cell shape, abnormal
AB + MO4-six1b standard conditions Prim-5 fast muscle cell myofibril position, abnormal
AB + MO4-six1b standard conditions Prim-5 muscle pioneer amount, normal
AB + MO4-six1b standard conditions Prim-5 slow muscle cell myofibril morphology, normal
AB + MO4-six1b standard conditions Prim-5 slow muscle cell myofibril position, normal
AB + MO4-six1b standard conditions Prim-5 somite U-shaped, abnormal
AB + MO4-six1b standard conditions Prim-5 somite fast muscle cell disorganized, abnormal
AB + MO4-six1b standard conditions High-pec skeletal myofibril assembly disrupted, abnormal
AB + MO4-six1b standard conditions High-pec trunk musculature refractivity, abnormal
AB + MO5-six1b standard conditions Prim-5 somite U-shaped, abnormal
High-pec skeletal myofibril assembly disrupted, abnormal
High-pec trunk musculature refractivity, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 323(2), Bessarab, D.A., Chong, S.W., Srinivas, B.P., and Korzh, V., Six1a is required for the onset of fast muscle differentiation in zebrafish, 216-228, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.