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ZIRC
ZFIN ID: ZDB-FIG-081028-11
Korzh et al., 2008 - Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafish. BMC Developmental Biology   8(1):84 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 6 Role of circulation in liver development. (A-F) Left-lateral views: liver morphogenesis in live LiPan wild type (A, D), LiPan/clo-/- mutants (B, E) and LiPan/Tnnt2 morphants (C, F) at 80 hpf (A-C) and 120 hpf (D-F). Dorsal views of the liver region of the same embryos are shown as inserts in each panel and dash lines indicate the midline with the right side at the top. Livers in clo-/- mutants and Tnnt2 morphants are located more medial, lack the anterio-ventral and posterior expansion, and are significantly reduced in size compared to the livers in wild type sibling. The cardiac edema in both mutants and morphants is indicated by arrow. (G-I) Left-lateral confocal in vivo projections of liver of LiPan/Tg(fli1:EGFP)y1 larvae at 120 hpf in wild type (G), clo-/- mutant (H) and Tnnt2 morphant (I) backgrounds. In clo-/ mutants ECs are absent (H), whereas in tnnt2 morphants they are present only between hepatocytes of the outer layer (I). (J-L) High-resolution light micrographs of hepatic parenchyma of zebrafish larvae stained with H&E. In 120-hpf wild type sibling, hepatocyte tubules are separated by sinusoids containing erythrocytes (J); in contrast, in 120-hpf clo-/- mutant (K) and Tnnt2 morphant (L), hepatocytes are tightly connected to each other. Note two sinusoids separated by two rows of neighboring hepatocytes as defined by yellow lines. Abbreviations: ec, endothelial cells; e, ear; h, hepatocytes; l, liver; s, sinusoid. In all images, anterior is towards the left. Scale bars, 125 μm in (A-F) and 625 μm in (G-L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz15Tg/gz15Tg standard conditions Protruding-mouth liver IFL
Day 5 liver IFL
gz15Tg/gz15Tg + MO1-tnnt2a standard conditions Protruding-mouth liver IFL
Day 5 liver IFL
gz15Tg; y1Tg standard conditions Day 5 liver IFL
gz15Tg; y1Tg + MO1-tnnt2a standard conditions Day 5 liver IFL
npas4ls5/s5; gz15Tg/gz15Tg standard conditions Protruding-mouth liver IFL
Day 5 liver IFL
npas4ls5/s5; gz15Tg/gz15Tg; y1Tg standard conditions Day 5 liver IFL
EGFP gz15Tg; y1Tg standard conditions Day 5 hepatic sinusoid blood vessel endothelial cell IFL
gz15Tg; y1Tg + MO1-tnnt2a standard conditions Day 5 liver blood vessel endothelial cell IFL
npas4ls5/s5; gz15Tg/gz15Tg; y1Tg standard conditions Day 5 Not Detected liver blood vessel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg/gz15Tg standard conditions Protruding-mouth liver morphology, normal
Day 5 liver morphology, normal
gz15Tg/gz15Tg + MO1-tnnt2a standard conditions Protruding-mouth liver decreased size, abnormal
Day 5 hepatic sinusoid aplastic, abnormal
Day 5 liver decreased size, abnormal
gz15Tg; y1Tg standard conditions Day 5 liver morphology, normal
gz15Tg; y1Tg + MO1-tnnt2a standard conditions Day 5 hepatic sinusoid aplastic, abnormal
Day 5 liver decreased size, abnormal
npas4ls5/s5; gz15Tg/gz15Tg standard conditions Protruding-mouth hepatic sinusoid aplastic, abnormal
Protruding-mouth liver decreased size, abnormal
Day 5 hepatic sinusoid aplastic, abnormal
Day 5 liver decreased size, abnormal
npas4ls5/s5; gz15Tg/gz15Tg; y1Tg standard conditions Day 5 hepatic sinusoid aplastic, abnormal
Day 5 liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.