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ZIRC
ZFIN ID: ZDB-FIG-081028-10
Korzh et al., 2008 - Requirement of vasculogenesis and blood circulation in late stages of liver growth in zebrafish. BMC Developmental Biology   8(1):84 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Pec-fin

Fig. 5 Role of endothelia in liver development. (A-F) Left-lateral views: liver morphogenesis in live LiPan/Tg(fli1:EGFP)y1 larvae; wild type (A, D), LiPan/clo-/- mutants (B, E) and LiPan/Tnnt2 morphants (C, F) at 60 hpf (A-C) and 70 hpf (D-F). Dorsal views of the liver region of the same embryos are shown as inserts in each panel and dash lines indicate the midline with the right side at the top. Note that the liver in LiPan/clo-/- mutants is significantly reduced compared to that in controls and Tnnt2 morphants. The pericardial edemas in both clo-/- mutant and tnnt2 morphant are indicated by arrows (B, C, E, F). (G-I) Left-lateral confocal in vivo projections of liver of LiPan/Tg(fli1:EGFP)y1 larvae in wild type (G), clo-/- mutant (H) and Tnnt2 morphant (I) backgrounds. Abbreviations: bd, bile duct; dph, daisy pattern of hepatocytes; ec, endothelial cells; e, ear; h, hepatocytes; l, liver; s, sinusoid. In all images, anterior is towards the left. Scale bars, 125 μm in (A-F) and 625 μm in (G-I).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz15Tg/gz15Tg standard conditions Pec-fin liver IFL
gz15Tg/gz15Tg + MO1-tnnt2a standard conditions Pec-fin liver IFL
gz15Tg; y1Tg standard conditions Pec-fin liver IFL
gz15Tg; y1Tg + MO1-tnnt2a standard conditions Pec-fin liver IFL
npas4ls5/s5; gz15Tg/gz15Tg standard conditions Pec-fin liver IFL
npas4ls5/s5; gz15Tg/gz15Tg; y1Tg standard conditions Pec-fin liver IFL
EGFP gz15Tg; y1Tg standard conditions Pec-fin liver blood vessel endothelial cell IFL
gz15Tg; y1Tg + MO1-tnnt2a standard conditions Pec-fin liver blood vessel endothelial cell IFL
npas4ls5/s5; gz15Tg/gz15Tg; y1Tg standard conditions Pec-fin Not Detected liver blood vessel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg/gz15Tg standard conditions Pec-fin liver morphology, normal
gz15Tg/gz15Tg + MO1-tnnt2a standard conditions Pec-fin liver morphology, normal
Pec-fin pericardium edematous, abnormal
gz15Tg; y1Tg standard conditions Pec-fin liver morphology, normal
gz15Tg; y1Tg + MO1-tnnt2a standard conditions Pec-fin liver morphology, normal
Pec-fin pericardium edematous, abnormal
npas4ls5/s5; gz15Tg/gz15Tg standard conditions Pec-fin liver decreased size, abnormal
Pec-fin liver development arrested, abnormal
Pec-fin pericardium edematous, abnormal
npas4ls5/s5; gz15Tg/gz15Tg; y1Tg standard conditions Pec-fin liver decreased size, abnormal
Pec-fin liver blood vasculature aplastic, abnormal
Pec-fin liver development arrested, abnormal
Pec-fin liver hepatocyte morphology, abnormal
Pec-fin pericardium edematous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.