FIGURE

Fig. 1

ID

ZDB-FIG-081013-12

Publication

Nelson et al., 2008 - The developmental sequence of gene expression within the rod photoreceptor lineage in embryonic zebrafish

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Fig. 1

BrdU incorporation within the rod lineage in embryonic zebrafish. Embryos were injected with BrdU at 60 hpf and fixed at 75 hpf. A: Retinal cryosection showing distribution of BrdU in the circumferential germinal zone (cgz) and in radial arrays of cells (arrowhead) spanning the inner (inl) and outer nuclear layers (onl) in central retina; le, lens; rpe, retinal pigmented epithelium; gc, ganglion cell layer; v, ventral; d, dorsal. B,C: BrdU and rod opsin; Nomarski optics (B) merged with epifluorescent image (C); arrow indicates colabeled cell. D: BrdU and gnat2 colabeling showing BrdU-labeled cones in retinal periphery (arrow) and cells in the central onl labeled only for BrdU (arrowheads). Regions to the periphery of the thin white lines (located approximately 6 onl cell diameters from the unlaminated cgz) were excluded from subsequent analyses related to the rod lineage. E-G: BrdU and anti-GS; arrow indicates colabeled cell. H-J: BrdU and islet1; no colabeling was observed (arrowhead indicates singly-labeled cell). K-M: Section doubly-labeled for BrdU and PKC; no colabeling was observed (arrowhead indicates singly-labeled cell). Scale bar in A (applies to A, D) = 40 μm, in B (applies to B,C,E-M) = 10 μm.

Expression Data

Genes:	gnat2 isl1a rho
Antibodies:	Ab1-glul Ab1-prkc Ab2-BrdU
Fish:	WT
Condition:	chemical treatment: 5-bromo-2'-deoxyuridine
Anatomical Terms:	amacrine cell ciliary marginal zone Muller cell retinal inner nuclear layer retinal outer nuclear layer retinal rod cell rod bipolar cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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