FIGURE

Fig. 4

ID

ZDB-FIG-081010-6

Publication

Liu et al., 2008 - Generation of oocyte-specifically expressed cre transgenic zebrafish for female germline excision of loxp-flanked transgene

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Test of Cre-mediated recombination at the chromosome level by breeding of Tg(zp3:cre, krt8:rfp) and Tg(mylz2:loxP-EGFP-loxP)^gz3. A: The 48 hpf embryos with strong, weak, and nil green fluorescent protein (GFP) expression from a cross between female Tg(zp3:cre, krt8:rfp) and male Tg(mylz2:loxP-EGFP-loxP)^gz3. Percentages of each type of embryos based on GFP expression are indicated. B: Polymerase chain reaction (PCR) detection of Cre-mediated recombination in offspring of female double transgenic fish Tg(zp3:cre, krt8:rfp)/Tg(mylz2:loxP-EGFP-loxP)^gz3 and male wild-type fish. In both skin RFP-expressing (cre transgenic) and non-RFP-expressing (cre non-transgenic) embryos (4 days postfertilization), approximately half of them showed Cre-mediated recombination as anticipated from the female germline excision while the other half were loxP non-transgenic individuals. The expected PCR products are 2.1 kb and 1.2 kb, respectively, before and after the Cre-mediated recombination. P, positive PCR control with pMYLZ2-loxP-EGFP-loxP plasmid that is used to generate Tg(mylz2:loxP-EGFP-loxP)^gz3; N, negative PCR control without a template DNA; M, 1-kb DNA ladder. Scale bar = 500 μm.

Expression Data

Gene:	EGFP
Fish:	gz14Tg; gz3Tg
Anatomical Term:	muscle
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.