ZFIN ID: ZDB-FIG-081010-6
Liu et al., 2008 - Generation of oocyte-specifically expressed cre transgenic zebrafish for female germline excision of loxp-flanked transgene. Developmental dynamics : an official publication of the American Association of Anatomists   237(10):2955-2962 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Long-pec

Fig. 4 Test of Cre-mediated recombination at the chromosome level by breeding of Tg(zp3:cre, krt8:rfp) and Tg(mylz2:loxP-EGFP-loxP)gz3. A: The 48 hpf embryos with strong, weak, and nil green fluorescent protein (GFP) expression from a cross between female Tg(zp3:cre, krt8:rfp) and male Tg(mylz2:loxP-EGFP-loxP)gz3. Percentages of each type of embryos based on GFP expression are indicated. B: Polymerase chain reaction (PCR) detection of Cre-mediated recombination in offspring of female double transgenic fish Tg(zp3:cre, krt8:rfp)/Tg(mylz2:loxP-EGFP-loxP)gz3 and male wild-type fish. In both skin RFP-expressing (cre transgenic) and non-RFP-expressing (cre non-transgenic) embryos (4 days postfertilization), approximately half of them showed Cre-mediated recombination as anticipated from the female germline excision while the other half were loxP non-transgenic individuals. The expected PCR products are 2.1 kb and 1.2 kb, respectively, before and after the Cre-mediated recombination. P, positive PCR control with pMYLZ2-loxP-EGFP-loxP plasmid that is used to generate Tg(mylz2:loxP-EGFP-loxP)gz3; N, negative PCR control without a template DNA; M, 1-kb DNA ladder. Scale bar = 500 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gz14Tg; gz3Tg standard conditions Long-pec muscle IFL
gz14Tg; gz3Tg standard conditions Long-pec muscle IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.