Transient expression of pKRT8-LGLR in zebrafish embryos by co-injection of pCMV-CRE or pZP3-CRE. A: Schematic representation of chimeric DNA constructs, pKRT8-LGLR, pCMV-CRE, and pZP3-CRE. B: A 48 hours postfertilization (hpf) embryo injected with pKRT8-LGLR only, showing only GFP expression. C,D: A same 48 hpf embryo co-injection of pKRT8-LGLR and pCMV-CRE, showing both green fluorescent protein (GFP) expression under a blue light (C) and red fluorescent protein (RFP) expression under a yellow light (D). E,F: A same 48 hpf embryo co-injection of pKRT8-LGLR and pZP3-CRE, showing both GFP (E) and RFP (F) expression. Scale bars = 500 μm.
|