FIGURE

Fig. 3

ID

ZDB-FIG-081010-5

Publication

Liu et al., 2008 - Generation of oocyte-specifically expressed cre transgenic zebrafish for female germline excision of loxp-flanked transgene

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Fig. 3

Cre RNA expression in Tg(zp3:cre, krt8:rfp) transgenic zebrafish. A,B: cre RNA expression in 2-72 hours postfertilization (hpf) embryos (A) and in adult transgenic fish (B) as detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Embryonic stages in hpf (A) and tissue sources (B) are indicated at the top of each lane. Whole-male transgenic fish (WFB) and selected tissues from female transgenic fish were used for RNA preparation. P, positive control with pZP3-CRE plasmid; N, negative control without template. β-actin probes were used for RT-PCR control. C,D: Ovary expression of cre mRNA in Tg(zp3:cre, krt8:rfp) transgenic female (C) and zp3 mRNA wild-type female (D). Whole-mount in situ hybridization detection was performed with isolated ovary tissues and both cre and zp3 mRNAs were detected in developing oocytes (arrows) but not in the mature oocyte.

Expression Data

Gene:	Cre
Fish:	gz14Tg
Anatomical Term:	whole organism
Stage Range:	64-cell to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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