Fig. 5
- ID
- ZDB-FIG-080828-10
- Publication
- Dee et al., 2008 - Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm
- Other Figures
- (all 10)
- All Figure Page
- Back to All Figure Page
Sox3 expression is inhibited by MO injection. (A) Sequence of sox3 5′UTR with MO binding sites labelled. Start codon indicated in bold and the positions of control MO mis-matches are indicated by asterisks. (B) Sections taken from the head of 24 hpf embryos and stained with anti-Sox3 and DAPI. A profound reduction of Sox3 protein expression is seen in embryos co-injected with sox3MO1/2 as compared to wild-type controls. (C) Co-injection of sox3MO1/2 blocked translation of a co-injected sox3-GFP fusion containing the MO target sequence as assayed by GFP fluorescence examined at 50% epiboly but had no effect on GFP alone. (D) Extent of knock-down of GFP fusions assayed by Western blot to detect the GFP protein. Actin was used as a loading control for the Western blot. Injection of sox3MO1 or sox3MO2 individually can be seen to knock-down sox3 translation less effectively than when used in combination. Control MOs (sox3mmMO1/2) could not block translation of sox3-GFP. |
Gene: | |
---|---|
Antibody: | |
Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage: | Prim-5 |
Fish: | |
---|---|
Knockdown Reagents: | |
Observed In: | |
Stage: | Prim-5 |
Reprinted from Developmental Biology, 320(1), Dee, C.T., Hirst, C.S., Shih, Y.H., Tripathi, V.B., Patient, R.K., and Scotting, P.J., Sox3 regulates both neural fate and differentiation in the zebrafish ectoderm, 289-301, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.