FIGURE

Fig. 4

ID

ZDB-FIG-080730-17

Publication

Clément et al., 2008 - Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher

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Fig. 4

dak^-/- and pic^-/- larvae have wild-type levels of expression of markers of chondrocyte differentiation.

Wholemount RNA in situ analysis of sox9a (A–C), collagen2a1a (D–F), chondromodulin (G–I) and gdf5 (J–L) at 60hpf (all ventral views of the head). Although the position of the developing skeleton varies between wild-type and mutants, the markers are expressed at similar levels in wild-type (A,D,G,J), dak^-/- (B,E,H,K) and pic^-/- (C,F,I,L). dak^-/- larvae express sox9a at higher levels anteriorly, but this is perhaps due to more chondrogenic cells being present (see Figure 6). Expression of gdf5 in the perichondrium of the ceratohyal is present albeit slightly reduced in dak^-/- and pic^-/- (arrows in J–L). The perichondrium of the hyosymplectic is also seen in toluidine blue stained sections at day 5 (arrows in M,N,O). Panel I scale bar = 50μM. Panel L scale bar = 5μM.

Expression Data

Genes:	cnmd col2a1a gdf5 sox9a
Fish:	WT ext2^{to273b/to273b} slc35b2^{to14mx/to14mx}
Anatomical Terms:	ceratohyal cartilage chondrocyte perichondrium
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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