FIGURE

Fig. 2

ID

ZDB-FIG-080730-15

Publication

Clément et al., 2008 - Regulation of zebrafish skeletogenesis by ext2/dackel and papst1/pinscher

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Fig. 2

Sulphated proteoglycans are reduced in dak^-/- and pic^-/- larvae.

Whole mount antibody staining at 24hpf reveals that HS is reduced in the somites of dak^-/- and pic^-/- (A–C) and KS in the notochord is reduced only in pic^-/- larvae (D–F). Cartilage staining of the ceratohyal at 72hpf reveals that CS is made in wild-type and dak^-/- chondrocytes (red stain in G,H), and absent from pic^-/- cartilage (I) (nuclear staining is shown in blue with DAPI). HPLC analysis of HS in pic^-/- larvae at day 5 indicates that sulphated disaccharides are nearly absent and unsulphated disaccharides are reduced (red) compared to their siblings (blue) (J). 1: ΔUA-GlcNAc, unsulfated Δ^4,5-unsaturated hexuronate -N-acetyl glucosamine; 2: ΔUA-GlcNS, ΔUA-N-sulfated glucosamine; 3: ΔUA-GlcNAc6S, ΔUA-6-O-sulfated GlcNAc; 4: ΔUA-GlcNS6S, ΔUA-N-sulfated, 6-O-sulfated glucosamine; 5: ΔUA2S-GlcNS, 2-O-sulfated ΔUA-N-sulfated glucosamine; 6: ΔUA2S-GlcNS6S, 2-O-sulfated ΔUA-N-sulfated, 6-O-sulfated glucosamine. These disaccharides correspond to the major disaccharides found in both invertebrate and vertebrate animals. Panel F scale bar = 50μM. Panel I scale bar = 10μM.

Expression Data

Antibodies:	Ab-3H1 Ab-10E4 Ab-CS-56
Fish:	WT ext2^{to273b/to273b} slc35b2^{to14mx/to14mx}
Anatomical Terms:	chondrocyte notochord somite
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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