Tdrd7 function is required for proper germ cell granule architecture. A) Tdrd7-GFP fusion protein colocalizes with the germ cell granule marker Vasa-dsRed. A 3-somite stage embryo corresponds to 11 hpf embryo. B) Tdrd7 function is required the formation of uniform normal sized granules. A 3D projection of granules labeled with Vasa antibody in 24 hours old control cells (left panel), in Tdrd7 depleted cells (middle) and in cells from embryos co-injected with the antisense oligonucleotide directed against Tdrd7 and an RNA encoding Tdrd7 that is resistant for the inhibition by the antisense oligonucleotide (right). C) Size of the largest granule in 24 hpf PGC. N = 18 cells for control, 16 for morpholino 1 injected embryos (T-test compared with the control, p < 10-3), 19 for morpholino 2 and 16 cells in which the phenotype was reversed by co-injection of the morpholino resistant Tdrd7 RNA. D) Number of granules in 24 hpf PGC. N = 13 cells for control, 19 cells for morpholino 1 (T-test compared with the control, p < 10-2), 26 cells for morpholino 2 and 13 cells in which the phenotype was reversed by co-injection of the morpholino resistant Tdrd7 RNA., TTest p < 0,003). E) Granule-size distribution is increased in cells knocked down for Tdrd7 as the proportion of the very small and very large granules is increased. N = 223 granules for control, 150 granules for morpholino 1 and 132 granules in which the phenotype was reversed by co-injection of the morpholino resistant Tdrd7 RNA.
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