FIGURE

Fig. 3

ID
ZDB-FIG-080617-25
Publication
Kishimoto et al., 2008 - Cystic kidney gene seahorse regulates cilia-mediated processes and Wnt pathways
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

seahorse and Wnt Signaling

(A) Wild-type and seahorse morphants at early segmentation stage. All are side views with anterior to the top and dorsal to the right. Class I has mild precocious tail extension. Class II has severe tail extension but with eyecups. Class III has severe tail extension and reduction of eye cups.

(B) Embryos injected with indicated combination of morpholino oligos and mRNA from three independent experiments. *p < 0.05.

(C and D) Side view of chordin expression at late-shield stage. Dorsal is to the right.

(E–J) Animal pole views with dorsal to the right. (E and F) Expression of gsc at 30% epiboly. (G–J) Expression of gsc and vent at shield stage. Ctr, embryos injected with the control morpholino; mph, embryos injected with sea-MO.

(K) Embryos injected with 1 ng sea-MO (sea), 0.1 ng prickle 1 (pk1) morpholino and 1 ng inversin (inv) morpholino in different combinations as shown in the figure. Left panel shows embryos on 1 dpf in side views with head to the left. Right panel shows in situ for krox20 and myoD on embryos fixed at the early segmentation stage, all are flat-mounted in dorsal views with head to the left.

(L) Tabulation of embryos with body curvature and short body axis phenotypes. The “short” phenotype refers to embryos with only residual tail extension or residual yolk extension. *p < 0.001 for sea + pk1 (n = 3), sea + inv (n = 3), and inv + pk1 (n = 3) when compared with samples injected with sea (n = 4), pk1 (n = 4), or inv (n = 6) morpholino alone.

(M) Paraxial cells labeled with membrane-anchored eGFP. Embryos are in dorsal views with anterior to the top and midline to the right. Left panel shows an embryo injected with control morpholino (C), while right panel shows an embryo injected with 2 ng sea-MO.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 30%-epiboly to 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 30%-epiboly to Prim-5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 14(6), Kishimoto, N., Cao, Y., Park, A., and Sun, Z., Cystic kidney gene seahorse regulates cilia-mediated processes and Wnt pathways, 954-961, Copyright (2008) with permission from Elsevier. Full text @ Dev. Cell