Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-FIG-080604-61
Zhang et al., 2008 - Cell cycle progression is required for zebrafish somite morphogenesis but not segmentation clock function. Development (Cambridge, England)   135(12):2065-2070 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. S1 Somite morphogenesis, but not somite polarity, is aberrant in emi1 mutant embryos. (A,B) Lateral views of (A) wild-type and (B) emi1-/- embryos at the 16- to 18-somite stage. (C,D) Lateral view of (C) deltaC morpholino-injected emi1-/- embryos and (D) deltaD morpholino-injected emi1-/- embryos at the 14- to 15-somite stage. (E) An aphidicolin/hydroxyurea-treated embryo at 30 hpf. (F) her1 stripe integrity was examined in emi1-/- and sibling embryos injected with morpholinos against either deltaC or deltaD. her1 expression was rated according to four categories representing increasing levels of disorganization: wild type, stripes with some salt and pepper expression, stripes begin to merge, and no stripes. Distributions of gene expression patterns are displayed for three independent trials (x-axis). Within each gene expression category, the fraction of sibling embryos is subtracted from the fraction of mutant embryos. For example, the wild-type category in the first deltaC morpholino trial included 0.28 fraction of the mutant embryos (28%) and 0.03 fraction of the sibling embryos (3%), giving a graphed value of 0.25. In deltaC morpholino trials, the number of mutants and siblings assayed (mutant/sib) are: 39/74, 27/79 and 16/57. For deltaD morpholino trials, the corresponding numbers are: 60/50, 28/74 and 49/90. These embryos were assayed in Fig. 3N, but here they were examined by a second investigator in a blind trial. (G,H) mesogenin expression in the tailbud of (G) wild-type and (H) emi1-/- embryos at the 15-somite stage. The expression domain is reduced in the mutant. (I,J) mespb expression in the anterior PSM of (I) wild-type and (J) emi1-/- embryos at the 12-somite stage. (K,L) ripply1 expression in (K) wild-type and (L) emi1-/- embryos at the 12-somite stage. (M,N) tbx18 expression in (M) wild-type and (N) emi1-/- embryos at the 12-somite stage. Segmental expression of mespb, ripply1 and tbx18 is established in the mutant. In G-N, anterior is up. (O) Ephrin B2 localization in two wild-type and two emi1-/- embryos. Shown is the 2-9 somite region of 12-somite-stage embryos. Anterior is up. In both mutant and wild type, Ephrin B2 staining is stronger in the cells anterior to each somite border than in the row of cells posterior to each border.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mespba WT standard conditions 10-13 somites segmental plate ISH
fbxo5tiy121/tiy121 standard conditions 10-13 somites segmental plate ISH
msgn1 WT standard conditions 14-19 somites tail bud ISH
fbxo5tiy121/tiy121 standard conditions 14-19 somites tail bud ISH
ripply1 WT standard conditions 10-13 somites segmental plate ISH
fbxo5tiy121/tiy121 standard conditions 10-13 somites segmental plate ISH
tbx18 WT standard conditions 10-13 somites segmental plate ISH
fbxo5tiy121/tiy121 standard conditions 10-13 somites segmental plate ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fbxo5tiy121/tiy121 standard conditions 14-19 somites somite specification disrupted, abnormal
fbxo5tiy121/tiy121 + MO1-dlc standard conditions 14-19 somites somite specification disrupted, abnormal
fbxo5tiy121/tiy121 + MO2-dld standard conditions 14-19 somites somite specification disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development