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ZFIN ID: ZDB-FIG-080522-18
Hashimoto et al., 2000 - Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formation. Developmental Biology   217(1):138-152 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Sphere to 90%-epiboly

Fig. 5 Maintenance of dkk1 expression depends on bozozok/dharma and Nodal signals. (A) dkk1 expression in dorsal marginal blastoderm and dorsal YSL was detected at the sphere stage in both bozm168 heterozygous (left) and homozygous (right) embryos. dkk1 expression in the embryonic shield was observed at the shield stage in bozm168 heterozygous embryos (left), but could not be detected in bozm168 homozygous embryos (right). When 5 pg of dharma RNA was injected to one blastomere (located in the lateral position) of 8-cell stage embryos, about a half of the dharma RNA-injected embryos exhibited expansion of dkk1 expression in the dorsal side, and the rest of them exhibited an ectopic expression of dkk1 at the shield stage (lower panel). Animal pole views, dorsal to the right. (B) dkk1 expression was normal at the sphere stage in both sqtcz35 heterozygous (left) and homozygous (right) embryos. dkk1 expression in the embryonic shield was observed at the shield stage in sqtcz35 heterozygous embryos (left), but could not be detected in sqtcz35 homozygous embryos (right).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dkk1b dharmam168/+(AB) standard conditions Sphere blastodisc dorsal margin ISH
Sphere yolk syncytial layer dorsal region ISH
Shield shield ISH
dharmam168/m168(AB) standard conditions Sphere blastodisc dorsal margin ISH
Sphere yolk syncytial layer dorsal region ISH
Shield Not Detected shield ISH
ndr1cz35/+ standard conditions Sphere blastodisc dorsal margin ISH
Sphere yolk syncytial layer dorsal region ISH
Shield shield ISH
ndr1cz35/cz35 standard conditions Sphere blastodisc dorsal margin ISH
Sphere yolk syncytial layer dorsal region ISH
Shield Not Detected shield ISH
tdgf1tz257/+(AB/TU) standard conditions Sphere blastodisc dorsal margin ISH
Sphere yolk syncytial layer dorsal region ISH
Shield shield ISH
90%-epiboly prechordal plate ISH
tdgf1tz257/tz257(AB/TU) standard conditions Sphere blastodisc dorsal margin ISH
Sphere yolk syncytial layer dorsal region ISH
Shield Not Detected shield ISH
90%-epiboly Not Detected prechordal plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 217(1), Hashimoto, H., Itoh, M., Yamanaka, Y., Yamashita, S., Shimizu, T., Solnica-Krezel, L., Hibi, M., and Hirano, T., Zebrafish Dkk1 functions in forebrain specification and axial mesendoderm formation, 138-152, Copyright (2000) with permission from Elsevier. Full text @ Dev. Biol.