FIGURE

Fig. S2

ID

ZDB-FIG-080505-43

Publication

Bertrand et al., 2008 - CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S2

HSCs and neurons can be segregated by light scatter characteristics from cmyb:eGFP embryos. (A) Lateral view of a cmyb:eGFP embryo at 42 hpf. The spinal cord shows small round GFP⁺ cells, whereas larger and brighter cells are observed in the AGM region (dorsal side upwards, anterior towards the left). (B) cmyb:EGFP⁺ cells were analyzed by forward (FSC) and side (SSC) scatter characteristics. Cells were sorted by size (red gate, larger; green gate, smaller). (C) QPCR analysis shows that FSC^lo cells are highly enriched for NCAM-expressing cells, whereas FSC^hi cells are enriched for CD45-expressing cells.

Expression Data

Genes:	EGFP ncam1a ptprc
Fish:	zf169Tg
Anatomical Terms:	spinal cord ventral wall of dorsal aorta
Stage:	High-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development