Morphometry of the Hammerhead Phenotype The distance between the eyes and the distance from the middle of the eyes to the tip of the nose (as indicated in the left panel) was determined in 4 dpf embryos after staining with alcian blue (see Materials and Methods). The ratio of the distance to the tip of the nose and the distance between the eyes was determined and is plotted in the bar graph, depicted in the panel on the right. Four WT and ten Shp2-MO injected embryos were analyzed. There is a significant difference in ratios between the WT (1.87 ± 0.03) and Shp2 knockdown (1.40 ± 0.20), indicating that Shp2 knockdown indeed induced a hammerhead phenotype.
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