FIGURE

Fig. 5

ID

ZDB-FIG-080429-14

Publication

Park et al., 2002 - olig2 is required for zebrafish primary motor neuron and oligodendrocyte development

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Fig. 5

olig2 promotes primary motor neuron development. (A-C) Side views of 20-hpf embryos hybridized with isl2 probe. (A) Normal distribution of one to two primary motor neurons per ventral spinal cord (sc) hemisegment (arrows) and dorsal Rohon-Beard neurons (RB). (B) Embryo injected with olig2 MO lacked primary motor neurons but had Rohon-Beard neurons. (C) Embryo injected with olig2 mRNA. Excess primary motor neurons developed in ventral spinal cord (arrows), whereas Rohon-Beard neurons appeared normal. Apparent differences in Rohon-Beard cell number reflect normal variation and difference in depth of optical section. (D-F) Transverse sections of embryos hybridized with lim1 RNA probe. lim1-expressing cells appeared normal in embryos injected with olig2 MO (E) or olig2 mRNA (F). Scale bar, 20 μm for all panels.

Expression Data

Gene:	isl2a
Fish:	WT WT + MO1-olig2
Knockdown Reagent:	MO1-olig2
Anatomical Terms:	primary motor neuron Rohon-Beard neuron
Stage:	20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-olig2
Knockdown Reagent:	MO1-olig2
Observed In:	interneuron primary motor neuron Rohon-Beard neuron spinal cord spinal cord motor neuron differentiation
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 248(2), Park, H.-C., Mehta, A., Richardson, J.S., and Appel, B., olig2 is required for zebrafish primary motor neuron and oligodendrocyte development, 356-368, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.