Abnormalities in the fast and slow muscle morphogenesis in the ATG-MO injected embryo. (A) The structure of the myhc3 locus and the SAGm11D insertion. T2KSAG was integrated downstream of the myhc3 gene. White boxes indicate untranslated regions and blue boxes indicate coding regions. (B) A lateral view of GFP expression in the SAGm11D embryo at 30 hpf. (C) A lateral view of a 24 hpf embryo hybridized with the myhc3 probe. (D) A confocal image of fast muscle fibers in an SAGm11D embryo at 36 hpf. (E) A confocal image of fast muscle fibers in an SAGm11D embryo injected with ATG-MO at 36 hpf. (F–I) Immunostaining using the S58 antibody that specifically detects slow muscle fibers. Confocal images of a wild type embryo (F, G) and an ATG-MO injected embryo at 32 hpf (H, I). (F, H) Lateral views of the surface area. (G, I) The same samples were rotated on 90° to generate transverse views. (J, K) Confocal images of somitic cells and adaxial cells located adjacent to the notochord. Dorsal views of a wild type embryo (J) and an ATG-MO injected embryo at 15 hpf (K). The notochord is at the bottom. Asterisks indicate the positions of the somite boundaries. The adaxial cells that elongate to span the length of the somite were formed both in wild type and the ATG-MO injected embryos.
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