FIGURE

Fig. 9

ID

ZDB-FIG-080417-29

Publication

Kotani et al., 2008 - misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance

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Fig. 9

The mys-deficient cells decreased lamellipodia formation on Fibronectin. (A, B) The morphology of cells dissociated from 4 hpf embryos and incubated on Fibronectin for overnight. (A) Cells dissociated from wild type embryos. (B) Cells dissociated from the ATG-MO injected embryos. The embryos were injected with rhodamine-dextran (red). Lamellipodia formation is impaired in the ATG-MO injected cells. (C–H) Analysis of FAK activation. Wild type cells (C, F) and the ATG-MO injected cells (D, G) incubated on Fibronectin for overnight. (E, H) Wild type cells immediately after placed on Fibronectin. (C, D, E) Images of rhodamine-dextran. (F, G, H) Immunostaining using the anti-phosphorylated FAK antibody. FAK is activated in the ATG-MO injected cells. Scale bars show 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-prrc1
Knockdown Reagent:	MO1-prrc1
Observed In:	cell lamellipodium non-membrane spanning protein tyrosine kinase activity
Stage:	Sphere

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 316(2), Kotani, T., and Kawakami, K., misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance, 383-396, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.