Fig. S7
TH expression in prox1 MO/otp1 mRNA coinjected embryos. Ventral view in all panels. Anterior is up. Anti-TH antibody labels the PT and hypothalamic CA neurons at 36 hpf (boxed regions). (B) microinjection of prox1 MO lowers the number of TH-labelled CA neurons in the hypothalamus in comparison to the 36 hpf embryos injected with the same concentration of standard control morpholino oligonucleotide (A). (C) coinjection of prox1 MO and otp1 synthetic mRNA did not restore the normal TH phenotype. Scale bar indicates 20 μm. |
Gene: | |
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Fish: | |
Knockdown Reagent: | |
Anatomical Terms: | |
Stage: | Prim-25 |