FIGURE

Fig. 4

ID

ZDB-FIG-080411-7

Publication

Wolman et al., 2008 - Transient axonal glycoprotein-1 (TAG-1) and laminin-alpha1 regulate dynamic growth cone behaviors and initial axon direction in vivo

Other Figures

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Fig. 4

TAG-1 or laminin-α1 loss of function causes misdirected MLF axon outgrowth.(a, b) Images from timelapse sequence of MLF axon outgrowth in TAG1MO injected (a) and bal;Tg(pitx2c:gfp) (b) embryos. Ventral views, anterior to the left, midline is up. Asterisks denote the caudal-most nucMLF cell. The dashed red line marks the nucMLF centerline. Arrows indicate MLF axons that wander into surrounding tissue and do not converge within timelapse duration. Arrowheads label an MLF axon that extends into lateral tissue, but eventually converges. The time stamp shows hours: minutes. (c) Schematic representation defining on-track (red shaded region) versus off-track regions. (d, e) Quantification of the average percentage of time MLF axons grew off-track (d) and the average percentage of axons that initially emerged from their cell body in an off-track position (e). *P < 0.001, two sample binomial comparison versus uninjected. N equals the number of MLF axons. Scale bar = 25 μm.

Expression Data

Gene:	EGFP
Fish:	zy8Tg + MO1-cntn2 lama1^uw1/uw1; zy8Tg
Knockdown Reagent:	MO1-cntn2
Anatomical Terms:	medial longitudinal fasciculus nucleus of the medial longitudinal fasciculus synencephalon
Stage Range:	14-19 somites to 20-25 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zy8Tg + MO1-cntn2 lama1^uw1/uw1; zy8Tg
Knockdown Reagent:	MO1-cntn2
Observed In:	axon guidance medial longitudinal fasciculus
Stage Range:	14-19 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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